Book Details
Orange Code:56022
Paperback:406 pages
Publications:
Categories:
Sections:
1. Derivation of Human Embryonic Stem Cells in Standard and Chemically Defined Conditions2. Autogeneic Feeders for the Culture of Undifferentiated Human Embryonic Stem Cells in Feeder and Feeder-Free Conditions3. Microporous Membrane Growth Substrates for Embryonic Stem Cell Culture and Differentiation4. Culture of Rodent Spermatogonial Stem Cells, Male Germline Stem Cells of the Postnatal Animal5. Characterization of Human Amniotic Fluid Stem Cells and Their Pluripotential Capability6. Method to Isolate Mesenchymal-Like Cells from Wharton's Jelly of Umbilical Cord7. Isolation, Characterization, and Differentiation of Human Umbilical Cord Perivascular Cells (HUCPVCs)8. Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance9. Culture of Pluripotent Neural Epithelial Progenitor Cells from E9 Rat Embryo10. Primary and Multipassage Culture of Human Fetal Kidney Epithelial Progenitor Cells11. Isolation of Human Mesenchymal Stem Cells from Bone and Adipose Tissue12. Culture of Mesenchymal Stem/Progenitor Cells in Adhesion-Independent Conditions13. Purification and Long-Term Culture of Multipotent Progenitor Cells Affiliated with the Walls of Human Blood Vessels: Myoendothelial Cells and Pericytes14. Isolation and Culture of Colon Cancer Stem Cells15. Isolation and Establishment of Human Tumor Stem Cells16. Stem Cells from Cartilaginous and Bony Fish
Description:
The purpose of this volume is to provide a comprehensive resource for researchers in the fields of embyonic, fetal and adult stem cell biology to find methods for the purification, culture, and differentitation of these cell types, with the main emphasis on the maintenance of the stem cell phenotype in vitro. This volume will be the first to broadly cover multiple types of stem cell culture from different ages, organs and species. Authors will focus on the practical do's and don'ts of isolating and culturing these cell types, and feel free to use illustrative data or diagrams whereever this improves the comprehension of the reader. This should allow the reader to compare and contrast techniques and make this a standard reference for those in the field, or desiring to start stem cell culture.
* Describes techniques in stem cell research
* Delineates critical steps and potential pitfalls for each method
*covers specific procedures in dealing with Human Embryonic Stem Cells
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