Avian Embryology

1. Operations on Primitive Streak Stage Avian Embryos
2. Quail–Chick Transplantations
3. Other Chimeras: Quail–Duck and Mouse–Chick
4. Manipulations of Neural Crest Cells or Their Migratory Pathways
5. Embryo Slices and Strips: Guidance and Adhesion Assays in the Avian Embryo
6. Neural Crest, Sensory Neuron, and Muscle Cultures
7. Methods in Avian Embryology Experimental and Molecular Manipulation of the Embryonic Chick Limb
8. Cell Division, DiVerentiation, and Death in Avian Embryos
9. In Situ Hybridization Analysis of Chick Embryos in Whole-Mount and Tissue Sections
10. Vital Labeling of Embryonic Cells Using Fluorescent Dyes and Proteins
11. Time-Lapse Imaging of the Early Avian Embryo
12. Gain- and Loss-of-Function Approaches in the Chick Embryo
13. Manipulation and Electroporation of the Avian Segmental Plate and Somites In Vitro
14. Transposon-Mediated Stable Integration and Tetracycline-Inducible Expression of Electroporated Transgenes in Chicken Embryos
15. Generating Transgenic Quail using Lentiviruses
16. Gene Discovery: Macroarrays and Microarrays
17. Dissection of Chick Genomic Regulatory Regions
18. Computational Approaches to Finding and Analyzing cis-Regulatory Elements
19. Investigating Regulatory Factors and Their DNA Binding AYnities Through Real Time Quantitative PCR (RT-QPCR) and Chromatin Immunoprecipitation (ChIP) Assays

This revised edition will continue to serve as the most complete and up-to-date guide to the use of the avian embryo in studies of vertebrate development. It will include new approaches to analysis of the chick genome, gene knock-out studies using RNA interference, morpholinos, and other cutting edge techniques. As with the original edition, emphasis has been placed on providing practical guidance, highlighting potentials and pitfalls of all key cell biological and embryological techniques.  *fully revised second edition *organized into basic and advanced Methods *new section on Functional Genomics