Avian Influenza Virus

1. A Brief Introduction to the Avian Infl uenza Virus
2. Avian Infl uenza Virus Sample Types, Collection, and Handling
3. Avian Infl uenza Virus RNA Extraction from Tissue and Swab Materia
4. Type A Infl uenza Virus Detection and Quantitation by Real-Time RT-PCR
5. Detection and Identifi cation of the H5 Hemagglutinin Subtype by Real-Time RT-PCR
6. Avian Infl uenza Virus Isolation and Propagation in Chicken Eggs
7. Hemagglutination Assay for the Avian Infl uenza Virus
8. Hemagglutination-Inhibition Test for Avian Infl uenza Virus Subtype Identifi cation and the Detection and Quantitation of Serum Antibodies to the Avian Infl uenza Virus
9. Neuraminidase-Inhibition Assay for the Identifi cation of Infl uenza A Virus Neuraminidase Subtype or Neuraminidase Antibody Specifi city
10. Immunohistochemical Staining for the Detection of the Avian Infl uenza Virus in Tissues
11. Wild Bird Surveillance for the Avian Infl uenza Virus
12. Reverse Genetics of the Avian Infl uenza Virus
13. Evaluating the Cell-Mediated Immune Response of Avian Species to Avian Infl uenza Viruses
14. Measurement of Avian Cytokines with Real-Time RT-PCR Following Infection with the Avian Infl uenza Virus

With the growing global fear of a major pandemic, avian influenza (AI) virus research has greatly increased in importance. In Avian Influenza Virus, an expert team of researchers and diagnosticians examine the fundamental, yet essential, virological methods for AI virus research and diagnostics as well as some of the newest molecular procedures currently used for basic and applied research. They present exciting, cutting-edge new methods that focus both on studying the virus itself and on work with avian hosts, an area greatly lacking in research.