Salmonella: Methods and Protocols

1. Luminex® Multiplex Bead Suspension Arrays for the Detection and Serotyping of Salmonella spp
2. Quantitative Proteomic Identification of Host Factors Involved in the Salmonella typhimurium Infection Cycle
3. Determination of Antimicrobial Resistance in Salmonella spp
4. Red-Mediated Recombineering of Salmonella enterica Genomes
5. A Method to Introduce an Internal Tag Sequence into a Salmonella Chromosomal Gene
6. Generation and Use of Site-Directed Chromosomal cyaA′ Translational Fusions in Salmonella enterica
7. Detection of Antimicrobial (Poly)Peptides with Acid Urea Polyacrylamide Gel Electrophoresis Followed by Western Immunoblot
8. Detecting Non-typhoid Salmonella in Humans by Enzyme-Linked Immunosorbent Assays (ELISAs): Practical and Epidemiological Aspects
9. Study of the Stn Protein in Salmonella; A Regulator of Membrane Composition and Integrity
10. Development of a Bacterial Nanoparticle Vaccine
11. Direct Attachment of Nanoparticle Cargo to Salmonella typhimurium Membranes Designed for Combination Bacteriotherapy Against Tumors
12. Applications of Microscopy in Salmonella Research
13. Live Cell Imaging of Intracellular Salmonella enterica
14. In Vitro Modeling of Gallbladder-Associated Salmonella spp. Colonization
15. Salmonella Phages and Prophages: Genomics, Taxonomy, and Applied Aspects

Salmonella: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed methods on different aspects and landmarks advancing knowledge on salmonella research. With new chapters on molecular assays for detection, identification and serotyping of salmonella, quantitative proteomic identification of host factors involved in salmonella infection, determination of antimicrobial resistance in salmonella, site-directed mutagenesis, chromosomal gene analysis, development of bacterial nanoparticle vaccine, attachment of nanoparticle cargo to biotinylated salmonella for combination bacteriotherapy against tumors, various microscopy methods to analyze salmonella interaction with host cells, in vitro modeling of gallbladder-associated salmonella colonization, and analysis of salmonella phages and prophages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Salmonella: Methods and Protocols, Second Edition present methods that are of value to investigators in the salmonella field.