Book Details
Orange Code:95555
Paperback:319 pages
Publications:
Categories:
Sections:
1. Animal Coronaviruses2. Feline Coronavirus: Insights into the Pathogenesis and Diagnosis3. Animal Models for Studying COVID-19 Pathology and Infection4. Isolation of Porcine Epidemic Diarrhea Virus from Clinical Samples5. Isolation and Characterization of Porcine Epidemic Diarrhea Virus Using Suspension Vero Cells6. Isolation and Propagation of Porcine Deltacoronavirus in Cell Culture and Embryonated Chicken Eggs7. Isolation and Propagation of Infectious Bronchitis Virus (Avian Coronavirus) in Chicken Embryonated Eggs8. Real-Time Reverse Transcription-Polymerase Chain Reaction for Simultaneous Detection of Bovine Coronavirus and Othe...9. TaqMan Real-Time RT-PCR Detection of Porcine Epidemic Diarrhea Virus10. Real-Time RT-PCR for Detection of Feline Coronavirus11. SARS-CoV-2 Real-Time RT-PCR Assay in Animals12. Real-Time PCR and Antigen-Capture ELISA for the Diagnosis of Porcine Deltacoronavirus Infection13. Identification of a Neutralizing Epitope-Containing Segment in Turkey Coronavirus Spike Protein for Diagnostic Ser...14. Antibody-Capture Enzyme-Linked Immunosorbent Assay for Detecting Protective Antibodies to Turkey Coronavirus Based...15. ELISA, Virus Neutralization, and Immunofluorescence Assays for Detection of Antibodies Against Porcine Deltacorona...16. Luminescent Immunoprecipitation System (LIPS) Development for the Specific Detection of PDCoV and SARS-CoV-2 Antib...17. Construction of a Mouse-Feline Chimeric Neutralizing Antibody Against SARS-CoV-218. Microneutralization Test of Avian Infectious Bronchitis Virus Using Vero Cells19. Pig Bioassay to Assess Infectivity of Porcine Epidemic Diarrhea Virus Detected in Various Sample Matrices20. Diagnostic Investigation and Confirmation of Emerging Turkey Coronaviral Enteritis21. Whole Genome Sequencing of Porcine Epidemic Diarrhea Virus and Porcine Deltacoronavirus22. Whole Genome Sequencing of Avian Infectious Bronchitis Virus by iSeq100 Platform and MinION23. Phylogenetic and Recombination Analysis of Animal Coronaviruses
Description:
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This detailed new edition compiles fully updated practical methodologies and approaches for diagnosticians and researchers working toward a better understanding of animal coronaviruses. The book explores vital techniques in virus isolation, diagnostic approaches via the detection of viral nucleic acids or proteins, serology, disease-targeted animal species models, and next-generation sequencing and bioinformatics. Written for the invaluable Springer Protocols Handbooks series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls.
Thorough and hands-on, Animal Coronaviruses, Second Edition will help readers design and carry out their increasingly important projects in clinical research, diagnostics, disease surveillance, and epidemiological study involving this family of viruses.
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