Techniques in Aquatic Toxicology, Volume 2

1. Integrative measures of toxicant exposure in zebra fish (Danio rerio) at different levels of biological organization
2. Use of disease challenge assay to assess immunotoxicity of xenobiotics in fish
3. Enhanced frog embryo teratogenesis assay: Xenopus model using Xenopus tropicalis
4. A short-term mummichog (Fundulus heteroclitus) bioassay to assess endocrine responses to hormone-active compounds and mixtures
5. Conducting dose–response feeding studies with salmonids: Growth as an endpoint
6. Field experiments with caged bivalves to assess chronic exposure and toxicity
7. Application of computer microscopy for histopathology in isopod toxicity studies
8. Sperm cell and embryo toxicity tests using the sea urchin Paracentrotus lividus (LmK)
9. Assessment of metal toxicity to sulfate-reducing bacteria through metal concentration methods
10. Cellular diagnostics and its application to aquatic and marine toxicology
11. A non-destructive technique to measure cytochrome P4501A enzyme activity in living embryos of the estuarine fish
12. Determination of lipid classes and lipid content in tissues of aquatic organisms using a thin layer chromatography/flame ionization detection (TLC/FID) microlipid method
13. Larval molting hormone synthesis and imaginal disc development in the midge Chironomus riparius as tools for assessing the endocrine modulating potential of chemicals in aquatic insects
14. The electro-olfactogram: An in vivo measure of peripheral olfactory function and sublethal neurotoxicity in fish
15. Enzyme-linked immunosorbent assay for screening estrogen receptor binding activity
16. Lysosomal destabilization assays for estuarine organisms
17. IMCOMP-P: An assay for coral immuno-competence
18. Monitoring gene expression in Rana catesbeiana tadpoles using a tail fin biopsy technique and its application to the detection of environmental endocrine disruptor effects in wildlife species
19. Coral reproduction and recruitment as tools for studying the ecotoxicology of coral reef ecosystems
20. Using the stickleback to monitor androgens and anti-androgens in the aquatic environment
21. Simple methods for estimating exposure concentrations of pesticide resulting from non-point source applications in agricultural drainage networks
22. Design and analysis of toxicity tests for the development and validation of biotic ligand models for predicting metal bioavailability and toxicity
23. Rapid toxicity fingerprinting of polluted waters using lux-marked bacteria
24. Aquatic in situ bioassays to detect agricultural non-point source pesticide pollution: A link between laboratory and field.
25. Improvements to high-performance liquid chromatography/ photodiode array detection (HPLC/PDA) method that measures dioxin-like polychlorinated biphenyls and other selected organochlorines in marine biota
26. Estrogenic activity measurement in wastewater using in vitro and in vivo methods
27. A toxicity assessment approach for evaluation of in-situ bioremediation of PAH contaminated sediments
28. Application of solid-phase microextraction fiber

Whether considering toxicant exposure in zebrafish, or the application of cellular diagnostics to marine toxicology, or the ecotoxicology of coral reef ecosystems, or the amount of metalloids in water, this reference offers the protocols for specimen collection that researchers need. Following up on his popular Techniques in Aquatic Toxicology with a second volume, now nine years later, Dr. Ostrander has once again called on the top aquatic toxicologists from across the world to present 39 chapters of unique collection and testing procedures.  Updating five techniques from the first volume, the authors have gone on to add over two dozen new techniques. Like the first volume, this text divides the chapters into four broad areas: Techniques for the Assessment of Toxicity in Whole Organisms, Cellular and Subcellular Toxicity, Contaminant Identification, and Impacts in Aquatic Ecosystems, and ends with a General Techniques section that anyone working in the field should find useful. Every chapter covers a specific procedure that can easily be reproduced by any competent technician with basic knowledge. Each of the chapter authors provides and interprets typical as well as anomalous results, false positives, and artifacts. Data is provided either from recently published experiments or from work being published for the first time.