Animal Influenza Virus

1. Hemagglutination Assay for Influenza Virus
2. Hemagglutination-Inhibition Assay for Influenza Virus Subtype Identification and the Detection and Quantitation of Serum Antibodies to Influenza Virus
3. Neuraminidase-Inhibition Assay for the Identification of Influenza A Virus Neuraminidase Virus Subtype or Neuraminidase Antibody Specificity
4. Reverse Genetics of Influenza Virus
5. Immunohistochemical Staining of Influenza Virus in Tissues
6. A Brief Introduction to Avian Influenza Virus
7. Wild Bird Surveillance for Avian Influenza Virus
8. Avian Influenza Virus Sample Types, Collection, and Handling
9. Avian Influenza Virus RNA Extraction
10. Avian Influenza Virus Detection and Quantitation by Real-Time RT-PCR
11. Influenza Subtype Identification with Molecular Methods
12. Avian Influenza Virus Isolation, Propagation, and Titration in Embryonated Chicken Eggs
13. Agar Gel Immunodiffusion Assay to Detect Antibodies to Type A Influenza Virus
14. Detection of Influenza A Antibodies in Avian Serum Samples by ELISA
15. Conducting Influenza Virus Pathogenesis Studies in Avian Species
16. Laboratory Methods for Assessing and Licensing Influenza Vaccines for Poultry
17. Detection of Cell-Mediated Immune Response to Avian Influenza Viruses
18. Characterization of Cytokine Expression Induced by Avian Influenza Virus Infection with Real-Time RT-PCR
19. Newcastle Disease Virus Detection and Differentiation from Avian Influenza
20. A Brief Introduction to Influenza A Virus in Swine
21. Sample Types, Collection, and Transport for Influenza A Viruses of Swine
22. Isolation of Swine Influenza Virus in Cell Cultures and Embryonated Chicken Eggs
23. RNA Extraction from Swine Samples and Detection of Influenza A Virus in Swine by Real-Time RT-PCR
24. Hemagglutinin Inhibition Assay with Swine Sera
25. Enzyme-Linked Immunosorbent Assay for Detection of Serum or Mucosal Isotype-Specific IgG and IgA Whole-Virus Antibody to Influenza A Virus in Swine
26. Serum Virus Neutralization Assay for Detection and Quantitation of Serum-Neutralizing Antibodies to Influenza A Virus in Swine
27. Microneutralization Assay for Swine Influenza Virus in Swine Serum
28. Analyzing Swine Sera for Functional Antibody Titers Against Influenza A Neuraminidase Proteins Using an Enzyme-Linked Lectin Assay (ELLA)
29. Antibody Secreting Cell Assay for Influenza A Virus in Swine
30. In Vivo Models for Pathotyping and Vaccine Efficacy for Swine Influenza
31. A Brief Introduction to Equine Influenza and Equine Influenza Viruses
32. Equine Influenza Diagnosis: Sample Collection and Transport
33. RNA Extraction from Equine Samples for Equine Influenza Virus
34. Type A Influenza Virus Detection from Horses by Real-Time RT-PCR and Insulated Isothermal RT-PCR
35. Equine Influenza Culture Methods
36. Equine Influenza Serological Methods

Avian Influenza Virus, Second Edition aims to provide the essential methods used in working with animal influenza viruses, and to compile more advanced information that will guide the user in designing influenza studies. Influenza A viruses are among the most important pathogens for humans, food animals and companion animals. Research and diagnostics with animal influenza viruses are critical to animal health and it should be recognized that the needs and goals of animal agriculture and veterinary medicine are not always the same as those of public health. This volume sorts out the differences in the structure of the poultry, swine and equine industries, the biological differences of influenza virus from each animal group, and provides host, strain and lineage specific guidance and procedures. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Avian Influenza Virus, Second Edition seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of these important pathogens.