Book Details
Orange Code:48528
Paperback:380 pages
Publications:
Categories:
Sections:
1. The Cell Culture Laboratory2. Sterilization3. Microscopy of Living Cells4. Basic Techniques and Media, the Maintenance of Cell Lines, and Safety5. Development and Optimization of Serum- and Protein-free Culture Media6. Cryopreservation and Banking of Cell Lines7. Primary Culture of Specific Cell Types and the Establishment of Cell Lines8. Cloning9. The Quality Control of Animal Cell Lines and the Prevention, Detection and Cure of Contamination10. Systems for Cell Culture Scale-up11. Good Laboratory Practice in the Cell Culture Laboratory
Description:
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This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable.
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