Molecular Dermatology Methods and Protocols

1. Molecular Dermatology Comes of Age
2. Molecular Diagnosis of Autoimmune Blistering Diseases
3. Molecular Diagnosis of Genodermatoses
4. Three-Dimensional Visualization of the Molecular Architecture of Cell–Cell Junctions In Situ by Cryo-Electron Tomography of Vitreous Sections
5. 3D Visualization of Epidermal Langerhans Cells
6. In Vivo Imaging of Lymph Node Lymphangiogenesis by Immuno-Positron Emission Tomography
7. Optical Imaging of HPV Infection in a Murine Model
8. Laser Scanning Microscopy Approach for Semiquantitation of In Vitro Dermal Particle Penetration
9. Analysis of Cutaneous Somatic Mosaicism
10. Global Proteome Analyses of SILAC-Labeled Skin Cells
11. Generation of Functional Multipotent Keratinocytes from Mouse Induced Pluripotent Stem Cells
12. RNAi-Mediated Gene Function Analysis in Skin
13. Mouse Models of Autoimmune Blistering Diseases Induced by the Passive Transfer of Antibodies
14. Induction of Experimental Epidermolysis Bullosa Acquisita by Immunization with Murine Collagen VII
15. Animal Model for Cutaneous Leishmaniasis
16. Model for Generation of Large Numbers of Primary, In fl ammatory Skin-Derived Neutrophils, and Macrophages
17. RNA Trans -Splicing for Genodermatoses

The sustained skin research efforts over the past decades has led to the accumulation of a significant collection of information on skin structure and physiology as well as on the pathogenesis of cutaneous diseases.  In Molecular Dermatology: Methods and Protocols, leading experts in the field provide a collection of state-of-the-art reliable protocols covering a wide spectrum of techniques and experimental models, specific molecular assays and disease models, as well as overviews of diagnostic and research areas relevant to molecular dermatology.  As a volume in the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Molecular Dermatology: Methods and Protocols emphasizes the vital importance of skin research and collects the methodologies necessary to aid scientists in moving forward in this valuable field.