Book Details
Orange Code:93445
Paperback:756 pages
Publications:
Categories:
Sections:
1. Introduction2. Biology of Cultured Cells3. Laboratory Design and Layout4. Equipment and Materials5. Aseptic Technique6. Safety, Bioethics, and Validation7. Culture Vessels and Substrates8. Defined Media and Supplements9. Serum-Free Media10. Preparation and Sterilization11. Supplementary Material12. Primary Culture13. Subculture and Cell Lines14. Authentication and Validation15. Microbial Contamination16. Cryopreservation and Banking17. Cloning and Selection18. Cell Sorting19. Cell Line Characterization20. Differentiation21. Three Dimensional Culture22. Scale up and Automation23. Senescence, Immortalization, and Transformation24. Quantitation25. Cytotoxicity26. Culture of Specific Cell Types27. Stem Cells28. Training Programs29. Problem Solving
Description:
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Since the publication of the sixth edition of this benchmark text, numerous advances in the field have been made – particularly in stem cells, 3D culture, scale-up, STR profiling, and culture of specialized cells. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols.This eagerly awaited edition reviews the increasing diversity of the applications of cell culture and the proliferation of specialized techniques, and provides an introduction to new subtopics in mini-reviews. New features also include a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling and barcoding, as well as some new specialized protocols. Because of the continuing expansion of cell culture, and to keep the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material online.Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. This text is an indispensable resource for those in or entering the field, including academic research scientists, clinical and biopharmaceutical researchers, undergraduate and graduate students, cell and molecular biology and genetics lab managers, trainees and technicians.
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