Understanding Enzymes Function, Design, Engineering, and Analysis

1. A Short Practical Guide to the Quantitative Analysis of Engineered Enzymes
2. Protein Conformational Motions: Enzyme Catalysis
3. Enzymology Meets Nanotechnology: Single-Molecule Methods for Observing Enzyme Kinetics in Real Time
4. Interfacial Enzyme Function Visualized Using Neutron, X-Ray, and Light-Scattering Methods
5. Folding Dynamics and Structural Basis of the Enzyme Mechanism of Ubiquitin C-Terminal Hydroylases
6. Stabilization of Enzymes by Metal Binding: Structures of Two Alkalophilic Bacillus Subtilases and Analysis of the Second Metal-Binding Site of the Subtilase Family
7. Structure and Functional Roles of Surface Binding Sites in Amylolytic Enzymes
8. Interfacial Enzymes and Their Interactions with Surfaces: Molecular Simulation Studies
9. Sequence, Structure, Function:What We Learn from Analyzing Protein Families
10. Bioinformatic Analysis of Protein Families to Select Function-Related Variable Positions
11. Decoding Life Secrets in Sequences by Chemicals
12. Role of Tunnels and Gates in Enzymatic Catalysis
13. Molecular Descriptors for the Structural Analysis of Enzyme Active Sites
14. Hydration Effects on Enzyme Properties in Nonaqueous Media Analyzed by MD Simulations
15. Understanding Esterase and Amidase Reaction Specificities by Molecular Modeling
16. Toward New Nonnatural TIM-Barrel Enzymes Using Computational Design and Directed Evolution Approaches
17. Handling the Numbers Problem in Directed Evolution
18. Hints from Nature: Metagenomics in Enzyme Engineering
19. A Functional and Structural Assessment of Circularly Permuted Bacillus circulans Xylanase and Candida antarctica Lipase B
20. Ancestral Reconstruction of Enzymes
21. High-Throughput Screening or Selection Methods for Evolutionary Enzyme Engineering
22. Nanoscale Enzyme Screening Technologies
23. Computational Enzyme Engineering: Activity Screening Using Quantum Chemistry
24. In Silico Screening of Enzyme Variants by Molecular Dynamics Simulation
25. Kinetic Stability of Variant Enzymes
26. index

Understanding Enzymes: Function, Design, Engineering, and Analysis focuses on the understanding of enzyme function and optimization gained in the past decade, past enzyme function analysis, enzyme engineering, and growing insights from the simulation work and nanotechnology measurement of enzymes in action in vitro or in silico. The book also presents new insights into the mechanistic function and understanding of enzyme reactions, as well as touching upon structural characteristics, including X-ray and nuclear magnetic resonance (NMR) structural methods. A major focus of the book is enzyme molecules’ dependency on dynamic and biophysical environmental impacts on their function in ensembles as well as single molecules. A wide range of readers, including academics, professionals, PhD and master’s students, industry experts, and chemists, will immensely benefit from this exclusive book.